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Journal: bioRxiv
Article Title: The mitochondrial transaminase GOT2 supports Japanese encephalitis virus replication in neuronal cells
doi: 10.64898/2026.10.12.758629
Figure Lengend Snippet: A) Viral RNA quantification in the brain. B) Heatmap showing the abundance profiles of proteins significantly altered in brain proteomics data (limma, adjusted p < 0.05) in mock versus infected samples at any of the six time points. Column annotations represent the experimental group and time point. Rows are proteins and are annotated with log2 fold-change values from differential abundance analysis at each time point. Cells corresponding to non-significant changes are shown as empty. Selected biologically relevant proteins are labeled. C ) Volcano plots of differential protein abundance at 6 dpi compared with mock samples. Key proteins are labeled. Non-significant proteins (adjusted p ≥ 0.05) are shown in grey. D ) Western blot analysis of JEV-NS1, GOT2 and ISG15 along with the HistineH3 as loading control. E ) Representative super-resolution TIRF microscopy image of infected brain tissue stained for MAP2 (red), JEV antigen (yellow) and IBA1 (cyan). Boxed regions i–iii are shown at higher magnification on the right. Scale bars, 50 µm in the overview image and 5 µm in magnified regions. F. Representative super-resolution TIRF microscopy image of infected brain tissue stained for MAP2 (red), JEV antigen (yellow) and GOT2 (green). Boxed regions i–iii are shown at higher magnification on the right. Scale bars, 50 µm in the overview image and 5 µm in magnified regions.
Article Snippet: The first cocktail included
Techniques: Infection, Analysis, Labeling, Quantitative Proteomics, Western Blot, Control, Super Resolution, Microscopy, Tissue, Staining